Micronucleus Test – in vivo
Gentronix now offers the micronucleus testing of rodent blood samples using flow cytometry
The rodent peripheral blood micronucleus test provides an assessment of the ability of a test compound to induce chromosomal damage or mis-segregation in haematopoietic cells in rodent bone marrow. During the generation of red blood cells, these progenitor stem cells differentiate to form erythroblasts, which in turn expel their nuclei to form immature red blood cells (reticulocytes, RETs). If these cells suffer chromosomal damage, nuclear membranes form around the resulting fragments, generating micronuclei. These micronuclei are not expelled with the nucleus of the erythroblast during differentiation, resulting in RETs that contain micronuclei and therefore DNA (MN-RETS).
Whilst MN-RETs from peripheral blood can be easily scored by microscopy, given the relative rarity of these events this is a time-consuming process that requires the scoring of thousands of reticulocytes per sample. Using a flow cytometric approach allows the robust & rapid enumeration of tens of thousands of RETs from a small aliquot of rodent blood. The flow cytometric rodent peripheral blood micronucleus test is included in the recent ICH revision to the guidelines on genotoxicity , with a requirement for restriction of analysis to the RETs population required for non-mouse in vivo studies – the offering from Gentronix meets this criteria. Results turnaround times are sample size dependent, although a typical 60 sample study completes within 2-weeks.
In addition to providing this service, Gentronix is also a distributor of the Litron Laboratory kits throughout Europe and Japan.