Assay Principles
The micronucleus assay detects aberrant, small nuclei present in the cell cytoplasm. Micronuclei may contain whole chromosomes ‘lost’ during mitosis or fragments generated by chromosome breakage. Usage of the micronucleus assay has increased in recent years – it is faster and less technical than the analysis of chromosomal aberrations. However, the traditional manual scoring method is laborious and so Litron Laboratories’ flow cytometric method is a significant advance. Flow cytometric assessment permits automated scoring, which removes subjectivity, and rapid measurement of large numbers of cells increases the statistical robustness of the results. In addition, the Litron method allows simultaneous collection of relative survival and membrane integrity parameters for cytotoxicity assessment, as well as cell cycle information. The service from Gentronix allows testing both in the presence and absence of exogenous metabolic activation using S9 fraction.
- Automated scoring, higher throughput
- Better data, faster results
- Multiple endpoint collection
- in vitro MicroFlow from Litron Laboratories
Positioning
The micronucleus testing service from Gentronix Ltd offers the opportunity for early identification of compounds likely to exhibit genotoxic liability. This allows prioritization of compounds for development, saving time and money. Use to provide mechanistic insight into earlier results such as, GreenScreen HC or as a bridge to the in vivo micronucleus test required in the ICH guidelines.
Click here to download a flyer for this service.
Please contact Gentronix for further details and to discuss your requirements for in vitro Micronucleus tests.