Assay Principles
The Comet assay, also called single cell gel electrophoresis, is a technique used for the sensitive detection of DNA damage in individual cells following exposure to a test compound. Electrophoresis is performed on exposed cells that have been embedded in agarose microgels. Damage resulting in DNA fragmentation is detectable by migration away from the nucleus. A DNA-stained damaged cell reveals the characteristic comet-like appearance: a bright ‘head’ (nucleus) and a ‘tail’ streak (damaged DNA). Types of damage measured include DNA strand breakage, DNA adducts and oxidative damage. The length of the comet ‘tail’ and the intensity of DNA staining within it are proportional to the extent of DNA damage.
- Sensitive DNA damage assessment
- Very low compound requirement
- Rapid turnaround, ideal for screening
- Reliable testing by genetic toxicology experts
Positioning
The Comet assay service from Gentronix Ltd offers the opportunity for early identification of compounds likely to exhibit genotoxic liability. This allows prioritization of compounds for development, saving time and money. Use to complement data from the ICH battery or to provide mechanistic insight into earlier results e.g. GreenScreen HC.
Click here to download a flyer for this service.
Click here to download details of the protocols followed and example data.
Please contact Gentronix for further details and to discuss your requirements for in vitro Comet assays.