Assay Principles
Ames MPF™ is an adapted microfluctuation method for performing the Ames bacterial mutagenicity test. Hisˉ or Trpˉ tester strains are incubated with test samples and scored for colony growth, indicating reversion to His+ or Trp+ phenotype has occurred. In this liquid format of the test, small cultures are scored in 384-well microplates using an indicator medium colour change (purple to yellow). A test agent is assayed across 6 dose levels with concurrent zero dose and positive controls, all performed in triplicate to provide statistical robustness. The test is operated both with and without S9 exogenous metabolism.
- 384-well microplate format bacterial mutation test
- Colorimetric read-out
- Low sample requirement
- OECD Guideline 471-based method
- Ames MPF™ assay protocol
Positioning
The Bacterial Mutagenicity testing service from Gentronix Ltd, offers the opportunity for early identification of compounds likely to produce positive results in the regulatory Ames mutagenicity test. This allows prioritization of compounds for development, saving time and money. The microplate format requires only the low, milligram quantities of test article that are available in discovery and development.
Click here to download a flyer for this service with example data.
Please contact Gentronix for further details and to discuss your requirements for bacterial mutagenicity screening.