GreenScreen HC, GreenScreen HC S9 and
BlueScreen HC
Raw absorbance and fluorescence data taken from the microplate reader is swiftly and automatically processed by the Excel based GreenScreen software provided with each kit. An example of the results output for a single microplate run using the GreenScreen assay is shown below. Four test compounds can be run in each 96 well microplate together with appropriate genotoxic and cytotoxic standard compounds and untreated controls. Each test chemical is usually run over nine twofold serial dilutions and the genotoxicity and cytotoxicity results are automatically processed, interpreted and displayed. The multi-layer output provides: (i) a single sheet summary of the genotoxicity and cytotoxicity results for all 4 compounds, plus the standard compound results for intra-plate quality control, (ii) detailed graphical results (dose responses) for each compound for user inspection, (iii) raw and processed numerical data for added transparency of the results.
Click on the image to download a PDF copy an example results sheet for GreenScreen HC.
The GreenScreen HC S9 assay data analysis template produces an output which is very similar to GreenScreen HC, presenting both qualitative and quantitative (dose response) data. The template processes the raw numerical data (GFP and propidium iodide fluoresecence and cell counts) exported from the flow cytometer.
The BlueScreen HC assay data analysis template also produces an output which is very similar to GreenScreen HC, presenting both qualitative and quantitative (dose response) data. The template processes the raw numerical data (optical absorbance, fluorescence and luminescence) exported from the microplate reader.
Please contact Gentronix to request copies of the GreenScreen HC and BlueScreen HC protocols and data interpretation guidence.